Conjugative Transfer and Expression of Genes Coding for Periplasmic Nitrate Reductase in the Purple Bacterium Rhodospirillum rubrum
نویسندگان
چکیده
Plasmid pFR400. a derivative of the vector plasmid pPHU231 containing the structural genes of the peri plasmic nitrate reductase (nap genes) of Rhodobacter sphaeroides DSM 158 (F. Reyes et al., Molec. Microbiol. 19, 1307-1318 [1996]) was transferred by conjugative mating to a streptomycin-resistant strain of the nitrate reductase-negative nonsulfur purple bacterium Rho dospirillum rubrum SI. Transconjugant cells of the latter bacterium, identified by their resistance to streptomycin and tetracycline and by their plasmid pattern, contained an active nitrate reductase which, like other periplasmic bacterial nitrate reductases, was not repressed by ammonium.
منابع مشابه
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